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Oxford location
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Edmund Cartwright House, 4 Robert Robinson Avenue
Oxford Science Park, Oxford, OX4 4GA, UK

Tel: +44 (0)845 034 7900 | Fax: +44 (0)845 034 7901

Cambridge location
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Suite 4, The Mansion, Chesterford Research Park
Little Chesterford, Essex, CB10 1XL, UK

Tel: +44 (0)845 034 7900 | Fax: +44 (0)845 034 7901

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If you have any enquires or questions, feel free to get in touch with Oxford Nanopore.

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Apply to the MAP

The MinION™ Access Programme (MAP) is a community-focused access project which started in Spring 2014. The philosophy of the MAP is to enable a broad range of people to explore how the MinION may be useful to them, to contribute to developments in analytical tools and applications and to share their experiences and collaborate. Listening to this community helps Oxford Nanopore provide continuous improvements to our products and support. To apply to join the MAP click here.

Protein analysis

The need for a new protein-analysis technology
Of the approximately 100,000 proteins commonly found in mammalian tissue, fewer than 5% have a reliable and affordable assay available. The development of reliable methods for analysing proteins is currently slow and complex. 

When identifying and verifying a new assay for a protein, mass spectrometry is likely to be the dominant technology. When progressing research into a protein to determine whether it is a useful biomarker for disease, or has other interesting functions, the researcher is likely to develop an immunoassay – again a long and complex process. In addition, many researchers use gene expression as a surrogate for protein analysis.

GridION™ technology for protein analysis
A direct electronic method of protein-analysis like nanopore sensing can provide benefits for researchers wishing to discover and validate new proteins. The same technology is designed to provide a diagnostic device with high specificity and sensitivity.
 

Oxford Nanopore is developing techniques for the direct, electronic analysis of proteins by combining nanopores with aptamers. An aptamer is a oligonucleic acid that can bind specifically to a site on a target protein. When used in the Oxford Nanopore technology platform, a bound aptamer-protein complex creates a characteristic disruption of the current running through a nanopore. It is desirable that this binding event should be reversible; the duration of a binding event provides further evidence of protein identity and the frequency of binding event provides information about concentration of that analyte.

This protein-analysis technique is compatible with the Oxford Nanopore GridION system.
 
The research below from Oxford Nanopore founder Professor Hagan Bayley's group illustrates the principle of this method, describing nanopore-ligand-protein interactions and the modification of a protein nanopore for the analysis of a protein kinase.
 
Stochastic detection of monovalent and bivalent protein-ligand interactions. Angewandte Chemie International Edition 43 (7), 842–846 (2004)
A genetically encoded pore for the stochastic detection of a protein kinase. ChemBioChem 7 (12), 1923–1927 (2006)