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Edmund Cartwright House, 4 Robert Robinson Avenue
Oxford Science Park, Oxford, OX4 4GA, UK

Tel: +44 (0)845 034 7900 | Fax: +44 (0)845 034 7901

Cambridge location
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Suite 4, The Mansion, Chesterford Research Park
Little Chesterford, Essex, CB10 1XL, UK

Tel: +44 (0)845 034 7900 | Fax: +44 (0)845 034 7901

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If you have any enquires or questions, feel free to get in touch with Oxford Nanopore.

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The MinION™ Access Programme (MAP) is a community-focused access project which started in Spring 2014. The philosophy of the MAP is to enable a broad range of people to explore how the MinION may be useful to them, to contribute to developments in analytical tools and applications and to share their experiences and collaborate. Listening to this community helps Oxford Nanopore provide continuous improvements to our products and support. To apply to join the MAP click here.

Publications from MinION Access Programme Participants
Successful test launch for nanopore sequencing. Nicholas J Loman, Mick Watson, Nature Methods 12, 303–304, doi:10.1038/nmeth.3327 (2015)   Improved data analysis for the MinION nanopore sequencer. Miten Jain, Ian T Fiddes, Karen H Miga, Hugh E Olsen, Benedict Paten & Mark Akeson Affiliations Contributions, Nature Methods 12, 351–356, doi:10.1038/nmeth.3290 (2015)
 

Bacterial and viral identification and differentiation by amplicon sequencing on the MinION nanopore sequencer. Andy Kilianski, Jamie L Haas, Elizabeth J Corriveau, Alvin T Liem, Kristen L Willis, Dana R Kadavy, C Nicole Rosenzweig and Samuel S Minot, GigaScience, 4:12, doi:10.1186/s13742-015-0051-z (2015)
 
 
Assessing the performance of the Oxford Nanopore Technologies MinION. T. Laver, J. Harrisona, P.A. O’Neill, K. Moorea,b, A. Farbosa, K. Paszkiewicza, D.J. Studholme, Biomolecular Detection and Quantification, Volume 3, 1-8 (2015)

A complete bacterial genome assembled de novo using only nanopore sequencing data. Nicholas J. Loman, Joshua Quick, Jared T. Simpson, bioRxiv, doi: 10.1101/015552 (2015)

        

Long read nanopore sequencing for detection of HLA and CYP2D6 variants and haplotypes. Ron Ammar, Tara A. Paton, Dax Torti, Adam Shlien, Gary D. Bader. F1000Research, doi: 10.12688/f1000research.6037.1 (2015)

Oxford Nanopore Sequencing and de novo Assembly of a Eukaryotic Genome. Sara Goodwin, James Gurtowski, Scott Ethe-Sayers, Panchajanya Deshpande, Michael Schatz, W Richard McCombie, bioRxiv, doi: http://dx.doi.org/10.1101/013490 (2015)

 

Nanopore Sequencing: From Imagination to Reality. Hagan Bayley. Clinical Chemistry, doi: 10.1373/clinchem.2014.223016 (2014)

MinION nanopore sequencing identifies the position and structure of a bacterial antibiotic resistance island. Philip M Ashton, Satheesh Nair, Tim Dallman, Salvatore Rubino, Wolfgang Rabsch, Solomon Mwaigwisya, John Wain  & Justin O'Grady. Nature Biotechnology, doi:10.1038/nbt.3103 (2014)

 

A reference bacterial genome dataset generated on the MinION™ portable single-molecule nanopore sequencer. Joshua Quick, Aaron R Quinlan & Nicholas J Loman. GigaScience, doi:10.1186/2047-217X-3-22 (2014)


poRe: an R package for the visualization and analysis of nanopore sequencing data. Mick Watson, Marian Thomson, Judith Risse, Richard Talbot, Javier Santoyo-Lopez, Karim Gharbi & Mark Blaxter. Bioinformatics, doi: 10.1093/bioinformatics/btu590 (2014)
 
 
Poretools: a toolkit for analyzing nanopore sequence data. Nicholas J. Loman & Aaron R. Quinlan. Bioinformatics, doi: 10.1093/bioinformatics/btu555 (2014)

 

 

Publications on nanopore sensing

The following publications have been generated by Oxford Nanopore Technologies or our academic collaborators.

Publications related to sequencing DNA and RNA

   

Strand sequencing

Error rates for nanopore discrimination among cytosine, methylcytosine, and hydroxymethylcytosine along individual DNA strands. Proceedings of the National Academy of Sciences of the United States of America 110 (47), 18910-5 (2013)

Kinetic mechanism of translocation and dNTP binding in individual DNA polymerase complexes. Journal  of the American Chemical Society 135 (24), 9149-55 (2013)

Single-stranded DNA within nanopores: conformational dynamics and implications for sequencing; a molecular dynamics simulation study. Biophysical Journal 103 (5), 1028-36 (2012)

Single molecule RNA base identification with a biological nanopore. Biophysical Journal 102 (3), 429a (2012)

Slowing down DNA translocation through a nanopore in lithium chloride. Nano Letters 12 (2), 1038–1044 (2012)

DNA base-calling from a nanopore using a Viterbi algorithm. Biophysical Journal Letters 102 (10), L37–L39 (2012)

Thermophoretic manipulation of DNA translocation through nanopores. ACS Nano 7 (1), 538–546 (2012)

Molecular dynamics simulations of DNA within a nanopore: arginine-phosphate tethering and a binding/sliding mechanism for translocation. Biochemistry 50 (18), 3777-83 (2011)

Processive replication of single DNA molecules in a nanopore catalyzed by ph29 DNA polymerase. Journal of the American Chemical Society 132 (50), 17961–17972 (2010)

        

           

          

 

Identification of epigenetic DNA modifications with a protein nanopore. Chemical Communications 46 (43), 8195–8197 (2010)

Replication of individual DNA molecules under electronic control using a protein nanopore. Nature Nanotechnology 5 (11), 798–806 (2010)

Nucleobase recognition in ssDNA at the central constriction of the alpha hemolysin pore. Nano Letters 10 (9), 3633–3637 (2010)

Multiple base recognition sites in a biological nanopore: two heads are better than one. Angewandte Chemie International Edition 49 (3), 556–559 (2010)

Single-nucleotide discrimination in immobilized DNA oligonucleotides with a biological nanopore. Proceedings of the National Academy of Sciences of the USA 106 (19), 7702–7707 (2009)

Sequence-specific detection of individual DNA strands using engineered nanopores. Nature Biotechnology 19 (7), 636–639 (2001)

Exonuclease sequencing

Continuous base identification for single-molecule nanopore DNA sequencing. Nature Nanotechnology 4 (4), 265–270 (2009)

Toward single molecule DNA sequencing. Journal of the American Chemical Society 128 (5), 1705–1710 (2006)

 

 

Publications related to protein analysis

   

Multistep protein unfolding during nanopore translocation. Nature Nanotechnology 8 (4), 288–295 (2013)

Unfoldase-mediated protein translocation through an α-hemolysin nanopore. Nature Biotechnology 31 (3), 247–250 (2013)

 

        

Protein detection by nanopores equipped with aptamers. Journal of the American Chemical Society 134 (5), 2781–2787 (2012)

Controlling protein translocation through nanopores with bio-inspired fluid walls. Nature Nanotechnology 6 (4), 253–260 (2011)

Publications related to nanopores as biosensors

   

Voltage-dependent properties of DNA origami nanopores. Nano Letters 14 (3), 1270-4 (2014)

Chitoporin from Vibrio harveyi, a channel with exceptional sugar specificity. The Journal of Biological Chemistry 288 (16), 11038-46 (2013)

Corynebacterium jeikeium jk0268 constitutes for the 40 amino acid long PorACj, which forms a homooligomeric and anion-selective cell wall channel. PLoS One 8 (10), e75651 (2013)

Lipid-bilayer-spanning DNA nanopores with a bifunctional porphyrin anchor. Angewandte Chemie (International edition in English) 52 (46), 12069-72 (2013)

Pulling peptides across nanochannels: resolving peptide binding and translocation through the hetero-oligomeric channel from Nocardia farcinica. ACS Nano 6 (12), 10699-707 (2012)

DNA origami nanopores. Nano Letters 12 (1), 512–517 (2012)

Single molecule detection of nitrogen mustards by covalent reaction within a protein nanopore. Journal of the American Chemical Society 130 (21), 6813–6819 (2008)

Protein Nanopores with covalently attached molecular adapters. Journal of the American Chemical Society 129 (151), 16142–16148 (2007)
 

 

Direct transfer of membrane proteins from bacteria to planar bilayers for rapid screening by single-channel recording. Nature Chemical Biology 2 (6), 314–318 (2006)

Stochastic sensing of enantiomers. Journal of the American Chemical Society 128 (33), 10684–10685 (2006)

A genetically encoded pore for the stochastic detection of a protein kinase. ChemBioChem 7 (12), 1923–1927 (2006)

Direct introduction of single protein channels and pores into lipid bilayers. Journal of the American Chemical Society 127 (18), 6502–6503 (2005)

Stochastic sensing of TNT with a genetically engineered pore. ChemBioChem 6 (10), 1875–1881 (2005)

Stochastic detection of monovalent and bivalent protein-ligand interactions. Angewandte Chemie International Edition 43 (7), 842–846 (2004)

Kinetics of a reversible covalent-bond forming reaction observed at the single-molecule level. Angewandte Chemie International Edition 41 (19), 3707–3709 (2002)

Capture of a single molecule in a nanocavity. Science 291 (5504), 636–640 (2001)

 

 

Publications related to solid-state nanopores

   

Pressure-controlled motion of single polymers through solid-state nanopores. Nano Letters 13 (7), 3048-52 (2013)

Molecule-hugging graphene nanopores. Proceedings of the National Academy of Sciences of the United States of America 110 (30), 12192-6 (2013)

Detection and quantification of methylation in DNA using solid-state nanopores. Scientific Reports 3, 1389 (2013)

Graphene quantum point contact transistor for DNA sensing. Proceedings of the National Academy of Sciences of the United States of America 110 (42), 16748–16753 (2013)

Electrochemistry at the edge of a single graphene layer in a nanopore. ACS Nano 7 (1), 834–843 (2013)

Stretching and controlled motion of single-stranded DNA in locally heated solid-state nanopores. ACS Nano 7 (8), 6816-24 (2013)

Assessing graphene nanopores for sequencing DNA. Nano Letters 12 (8), 4117–4123 (2012)

Translocating single-stranded DNA through crystalline graphene nanopores. Biophysical Journal 102 (1), 728a (2012)

Slowing down and stretching DNA with an electrically tunable nanopore in a p–n semiconductor membrane. Nanotechnology 23 (25), 255501 (2012)

 
A stacked graphene-Al2O3 nanopore architecture for DNA detection. Biophysical Journal 102 (3), 730a (2012)

Local electrical potential detection of DNA by nanowire-nanopore sensors. Nature Nanotechnology 7 (2), 119–125 (2012)

Lipid bilayer coated Al2O3 nanopore sensors: towards a hybrid biological solid-state nanopore. Biomed Microdevices 13 (4), 671-82 (2011)

Hybrid pore formation by directed insertion of alpha hemolysin into solid-state nanopores. Nature Nanotechnology 5 (12), 874–877 (2010)

Graphene as a sub nanometre trans-electrode membrane. Nature 467 (7312), 190–193 (2010)

Accompanying editorial by Professor Hagan Bayley

Ice lithography for nanodevices. Nano Letters 10 (12), 5056–5059 (2010)

Recapturing and trapping single molecules with a solid-state nanopore. Nature Nanotechnology 2 (12), 775–779 (2007)

DNA conformation and base number simultaneously determined in a nanopore. Electrophoresis 28 (18), 3186–3192 (2007)


 

Review articles

   

DNA sequencing with nanopores. Nature Biotechnology 30 (4), 326–328 (2012)

Nanopore analysis of nucleic acids bound to exonucleases and polymerases. Annual Review of Biophysics 39, 79–90 (2010)

The potential and challenges of nanopore sequencing. Nature Biotechnology 26 (10), 1146–1153 (2008)

Solid-state nanopores. Nature Nanotechnology 2 (4), 209–215 (2007)

 

Sequencing single molecules of DNA. Current Opinion in Chemical Biology 10 (6), 628–637 (2006)

Stochastic sensors inspired by biology. Nature 413 (6852), 226–230 (2001)

Stochastic sensing with protein pores. Advanced Materials 12 (2), 139–142 (2000)

Building doors into cells. Scientific American 277 (3), 62–67 (1997)