Introduction to nanopore sensing
The GridION™ system
- Sensor chip
The MinION™ device: a miniaturised sensing system
Analytes and applications: DNA, RNA, proteins
Fields of use
GridION™ nodes are designed to work with a single-use, self-contained cartridge that includes all the reagents required to run an experiment. The cartridge is compatible with standard laboratory multi-well plates.
All reagents are contained within the cartridge, and there are no reagents inside the instrument (nodes). The sample is introduced into the cartridge which is then inserted into the node. Cartridges are self-priming and no further user action is required once loaded.
A number of nanopores are recorded at any one time by using an arrayed sensor chip to create multiple individual recording channels. Signals from these channels are acquired by a specially designed Application-Specific Electronic Circuit (ASIC).
Adapting to automation and improved workflows
Multiple samples can be loaded into a cartridge via a multi-well plate manifold. This adaptation allows the node to analyse each sample in turn.
Coupled with the 'Run until…' functionality, each sample can be analysed until sufficient data has been gathered to satisfy the pre-determined experimental endpoint, and the node can then expel the sample, flush and move to the subsequent sample. Multi-well plate-based sample preparation can streamline and scale workflows for individual nodes or for clusters of nodes.
Adaptation of cartridges for different applications.
Cartridges may contain different types of nanopores so that they can analyse different types of molecule, e.g. DNA, RNA, proteins or small molecules. A different type of nanopore may be supplied for sub-types of these experiments, for example epigenetic analyses. Oxford Nanopore engineers different types of nanopore so that each is best suited to the analysis of a type of molecule; in DNA sequencing the Company plans to include more than one type of nanopore within a single chip, to provide complementary data and therefore enrich the analytical data.